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hap1 ifitm3 knockout  (ATCC)


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    ATCC hap1 ifitm3 knockout
    WT and <t>Ifitm3</t> −/− mice were intranasally infected with ( a – c ) 1, 10, or 50 TCID50 of H5N1 avian influenza strain (2 independent experiments for doses 1 and 10 ( n = 10 mice) and 1 experiment for dose of 50 ( n = 5 mice)) or with ( d – f ) 1 or 10 TCID50 of H7N3 avian influenza strain ( n = 5 mice). a , d Viral titers from lung homogenates at day 3 post infection. b , e ELISA quantification of IL-6 levels in lung homogenates at day 3 post infection. c , f ELISA quantification of IFNβ levels in lung homogenates at day 3 post infection. All error bars represent SEM. Comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Only comparisons between WT and Ifitm3 −/− mice for each dose are shown. a – f Each data point represents an individual mouse. The numbers shown above the graph represent exact p -values. Source data are provided as a Source Data file.
    Hap1 Ifitm3 Knockout, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hap1 ifitm3 knockout/product/ATCC
    Average 93 stars, based on 11 article reviews
    hap1 ifitm3 knockout - by Bioz Stars, 2026-03
    93/100 stars

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    1) Product Images from "Innate immune control of influenza virus interspecies adaptation via IFITM3"

    Article Title: Innate immune control of influenza virus interspecies adaptation via IFITM3

    Journal: Nature Communications

    doi: 10.1038/s41467-024-53792-3

    WT and Ifitm3 −/− mice were intranasally infected with ( a – c ) 1, 10, or 50 TCID50 of H5N1 avian influenza strain (2 independent experiments for doses 1 and 10 ( n = 10 mice) and 1 experiment for dose of 50 ( n = 5 mice)) or with ( d – f ) 1 or 10 TCID50 of H7N3 avian influenza strain ( n = 5 mice). a , d Viral titers from lung homogenates at day 3 post infection. b , e ELISA quantification of IL-6 levels in lung homogenates at day 3 post infection. c , f ELISA quantification of IFNβ levels in lung homogenates at day 3 post infection. All error bars represent SEM. Comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Only comparisons between WT and Ifitm3 −/− mice for each dose are shown. a – f Each data point represents an individual mouse. The numbers shown above the graph represent exact p -values. Source data are provided as a Source Data file.
    Figure Legend Snippet: WT and Ifitm3 −/− mice were intranasally infected with ( a – c ) 1, 10, or 50 TCID50 of H5N1 avian influenza strain (2 independent experiments for doses 1 and 10 ( n = 10 mice) and 1 experiment for dose of 50 ( n = 5 mice)) or with ( d – f ) 1 or 10 TCID50 of H7N3 avian influenza strain ( n = 5 mice). a , d Viral titers from lung homogenates at day 3 post infection. b , e ELISA quantification of IL-6 levels in lung homogenates at day 3 post infection. c , f ELISA quantification of IFNβ levels in lung homogenates at day 3 post infection. All error bars represent SEM. Comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Only comparisons between WT and Ifitm3 −/− mice for each dose are shown. a – f Each data point represents an individual mouse. The numbers shown above the graph represent exact p -values. Source data are provided as a Source Data file.

    Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

    a Schematic of in vitro infection with animal-origin influenza viruses and representative example flow cytometry dot plots from infected A549 human lung cells. b , e The indicated A549 cells or THP-1 differentiated macrophages were treated +/- IFNβ for 18 h followed by infection with the indicated viruses (MOI 1) for 24 h. Percent infection was determined by flow cytometry and normalized to respective control cells without IFNβ pre-treatment. Error bars represent SEM. Only statistical comparisons between shControl versus shIFITM3 and WT versus IFITM3 −/− are shown, determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data are representative of 3 independent experiments, each performed in triplicate ( n = 9). c , d Western blots of cell lysates at 18 h +/− IFNβ treatment. Note that commercial IFITM3 antibodies weakly detect IFITM2 in addition to IFITM3. The numbers shown above the graph represent exact p values. Source data are provided as a Source Data file. a Created in BioRender. Denz, P. (2024) BioRender.com/z17v580. Gating strategies for flow cytometry are depicted in Supplementary Fig. a. A549 cells and Supplementary Fig. b. THP-1 cells.
    Figure Legend Snippet: a Schematic of in vitro infection with animal-origin influenza viruses and representative example flow cytometry dot plots from infected A549 human lung cells. b , e The indicated A549 cells or THP-1 differentiated macrophages were treated +/- IFNβ for 18 h followed by infection with the indicated viruses (MOI 1) for 24 h. Percent infection was determined by flow cytometry and normalized to respective control cells without IFNβ pre-treatment. Error bars represent SEM. Only statistical comparisons between shControl versus shIFITM3 and WT versus IFITM3 −/− are shown, determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data are representative of 3 independent experiments, each performed in triplicate ( n = 9). c , d Western blots of cell lysates at 18 h +/− IFNβ treatment. Note that commercial IFITM3 antibodies weakly detect IFITM2 in addition to IFITM3. The numbers shown above the graph represent exact p values. Source data are provided as a Source Data file. a Created in BioRender. Denz, P. (2024) BioRender.com/z17v580. Gating strategies for flow cytometry are depicted in Supplementary Fig. a. A549 cells and Supplementary Fig. b. THP-1 cells.

    Techniques Used: In Vitro, Infection, Flow Cytometry, Control, Western Blot

    a Schematic of mouse passaging experiments. Initial intranasal infections were performed with 1000 TCID50 of parental viruses. b Schematic of WT mouse challenge with parental A/Victoria/361/2011 (H3N2) or passaged viruses. c – h Groups of WT mice ( n = 5 per group) were challenged with 1000 TCID 50 of A/Victoria/361/2011 (H3N2) virus passaged 1, 5, or 10 times through WT or Ifitm3 −/− mice and compared to the parent virus (passage 0). c , f Viral titers from lung homogenates collected at day 7 ( c represents 2 independent infections). d , g ELISA quantification of IL-6. e , h ELISA quantification of IFNβ. c – h Error bars represent SEM and comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Each dot represents an individual mouse. The numbers above the graph represent exact p -values. Source data are provided as a Source Data file. a , b Created in BioRender. Denz, P. (2024) BioRender.com/h97v852.
    Figure Legend Snippet: a Schematic of mouse passaging experiments. Initial intranasal infections were performed with 1000 TCID50 of parental viruses. b Schematic of WT mouse challenge with parental A/Victoria/361/2011 (H3N2) or passaged viruses. c – h Groups of WT mice ( n = 5 per group) were challenged with 1000 TCID 50 of A/Victoria/361/2011 (H3N2) virus passaged 1, 5, or 10 times through WT or Ifitm3 −/− mice and compared to the parent virus (passage 0). c , f Viral titers from lung homogenates collected at day 7 ( c represents 2 independent infections). d , g ELISA quantification of IL-6. e , h ELISA quantification of IFNβ. c – h Error bars represent SEM and comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Each dot represents an individual mouse. The numbers above the graph represent exact p -values. Source data are provided as a Source Data file. a , b Created in BioRender. Denz, P. (2024) BioRender.com/h97v852.

    Techniques Used: Passaging, Virus, Enzyme-linked Immunosorbent Assay

    Influenza virus A/California/04/2009 (H1N1) was passaged through mice as described in Fig. . Groups of WT mice ( n = 5 per group) were challenged with 1000 TCID 50 of A/California/04/2009 (H1N1) virus passaged 1, 5, or 10 times through WT or Ifitm3 −/− mice and compared to the parent virus (passage 0). a , e Viral titers from lung homogenates collected at day 7 ( a ) or day 6 ( e ) post infection. Error bars represent SEM, comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. b Weight loss for the H1N1 series 1 challenge. Error bars represent SEM, comparisons were made using the Mann-Whitney test. ELISA quantification of IL-6 ( c , g ) and IFNβ ( d , h ) levels in lung homogenates of WT and IFITM3 KO mice at day 7 ( c , d ) or day 6 ( g , h ) post infection. Error bars represent SEM and comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. f Weight loss for the H1N1 series 2 challenge. Skull and crossbones indicate humane euthanasia of all animals infected with KO passage 10. Error bars represent SEM, comparisons were made using the Mann-Whitney test. ( a , c , d , e , g , h ) Each dot represents an individual mouse. b , f dots represent averages of individual mice ( n = 5 per group). All numbers above the graphs represent exact p -values. Source data are provided as a Source Data file.
    Figure Legend Snippet: Influenza virus A/California/04/2009 (H1N1) was passaged through mice as described in Fig. . Groups of WT mice ( n = 5 per group) were challenged with 1000 TCID 50 of A/California/04/2009 (H1N1) virus passaged 1, 5, or 10 times through WT or Ifitm3 −/− mice and compared to the parent virus (passage 0). a , e Viral titers from lung homogenates collected at day 7 ( a ) or day 6 ( e ) post infection. Error bars represent SEM, comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. b Weight loss for the H1N1 series 1 challenge. Error bars represent SEM, comparisons were made using the Mann-Whitney test. ELISA quantification of IL-6 ( c , g ) and IFNβ ( d , h ) levels in lung homogenates of WT and IFITM3 KO mice at day 7 ( c , d ) or day 6 ( g , h ) post infection. Error bars represent SEM and comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. f Weight loss for the H1N1 series 2 challenge. Skull and crossbones indicate humane euthanasia of all animals infected with KO passage 10. Error bars represent SEM, comparisons were made using the Mann-Whitney test. ( a , c , d , e , g , h ) Each dot represents an individual mouse. b , f dots represent averages of individual mice ( n = 5 per group). All numbers above the graphs represent exact p -values. Source data are provided as a Source Data file.

    Techniques Used: Virus, Infection, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay



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    hap1 ifitm3 knockout  (ATCC)
    93
    ATCC hap1 ifitm3 knockout
    WT and <t>Ifitm3</t> −/− mice were intranasally infected with ( a – c ) 1, 10, or 50 TCID50 of H5N1 avian influenza strain (2 independent experiments for doses 1 and 10 ( n = 10 mice) and 1 experiment for dose of 50 ( n = 5 mice)) or with ( d – f ) 1 or 10 TCID50 of H7N3 avian influenza strain ( n = 5 mice). a , d Viral titers from lung homogenates at day 3 post infection. b , e ELISA quantification of IL-6 levels in lung homogenates at day 3 post infection. c , f ELISA quantification of IFNβ levels in lung homogenates at day 3 post infection. All error bars represent SEM. Comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Only comparisons between WT and Ifitm3 −/− mice for each dose are shown. a – f Each data point represents an individual mouse. The numbers shown above the graph represent exact p -values. Source data are provided as a Source Data file.
    Hap1 Ifitm3 Knockout, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hap1 ifitm3 knockout/product/ATCC
    Average 93 stars, based on 1 article reviews
    hap1 ifitm3 knockout - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

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    WT and Ifitm3 −/− mice were intranasally infected with ( a – c ) 1, 10, or 50 TCID50 of H5N1 avian influenza strain (2 independent experiments for doses 1 and 10 ( n = 10 mice) and 1 experiment for dose of 50 ( n = 5 mice)) or with ( d – f ) 1 or 10 TCID50 of H7N3 avian influenza strain ( n = 5 mice). a , d Viral titers from lung homogenates at day 3 post infection. b , e ELISA quantification of IL-6 levels in lung homogenates at day 3 post infection. c , f ELISA quantification of IFNβ levels in lung homogenates at day 3 post infection. All error bars represent SEM. Comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Only comparisons between WT and Ifitm3 −/− mice for each dose are shown. a – f Each data point represents an individual mouse. The numbers shown above the graph represent exact p -values. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Innate immune control of influenza virus interspecies adaptation via IFITM3

    doi: 10.1038/s41467-024-53792-3

    Figure Lengend Snippet: WT and Ifitm3 −/− mice were intranasally infected with ( a – c ) 1, 10, or 50 TCID50 of H5N1 avian influenza strain (2 independent experiments for doses 1 and 10 ( n = 10 mice) and 1 experiment for dose of 50 ( n = 5 mice)) or with ( d – f ) 1 or 10 TCID50 of H7N3 avian influenza strain ( n = 5 mice). a , d Viral titers from lung homogenates at day 3 post infection. b , e ELISA quantification of IL-6 levels in lung homogenates at day 3 post infection. c , f ELISA quantification of IFNβ levels in lung homogenates at day 3 post infection. All error bars represent SEM. Comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Only comparisons between WT and Ifitm3 −/− mice for each dose are shown. a – f Each data point represents an individual mouse. The numbers shown above the graph represent exact p -values. Source data are provided as a Source Data file.

    Article Snippet: HeLa IFITM1/2/3 knockout and HAP1 IFITM3 knockout were purchased from ATCC (CRL-3452) and Horizon Discovery Biosciences (HZGHC004186c010), respectively.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay

    a Schematic of in vitro infection with animal-origin influenza viruses and representative example flow cytometry dot plots from infected A549 human lung cells. b , e The indicated A549 cells or THP-1 differentiated macrophages were treated +/- IFNβ for 18 h followed by infection with the indicated viruses (MOI 1) for 24 h. Percent infection was determined by flow cytometry and normalized to respective control cells without IFNβ pre-treatment. Error bars represent SEM. Only statistical comparisons between shControl versus shIFITM3 and WT versus IFITM3 −/− are shown, determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data are representative of 3 independent experiments, each performed in triplicate ( n = 9). c , d Western blots of cell lysates at 18 h +/− IFNβ treatment. Note that commercial IFITM3 antibodies weakly detect IFITM2 in addition to IFITM3. The numbers shown above the graph represent exact p values. Source data are provided as a Source Data file. a Created in BioRender. Denz, P. (2024) BioRender.com/z17v580. Gating strategies for flow cytometry are depicted in Supplementary Fig. a. A549 cells and Supplementary Fig. b. THP-1 cells.

    Journal: Nature Communications

    Article Title: Innate immune control of influenza virus interspecies adaptation via IFITM3

    doi: 10.1038/s41467-024-53792-3

    Figure Lengend Snippet: a Schematic of in vitro infection with animal-origin influenza viruses and representative example flow cytometry dot plots from infected A549 human lung cells. b , e The indicated A549 cells or THP-1 differentiated macrophages were treated +/- IFNβ for 18 h followed by infection with the indicated viruses (MOI 1) for 24 h. Percent infection was determined by flow cytometry and normalized to respective control cells without IFNβ pre-treatment. Error bars represent SEM. Only statistical comparisons between shControl versus shIFITM3 and WT versus IFITM3 −/− are shown, determined by one-way ANOVA followed by Tukey’s multiple comparisons test. Data are representative of 3 independent experiments, each performed in triplicate ( n = 9). c , d Western blots of cell lysates at 18 h +/− IFNβ treatment. Note that commercial IFITM3 antibodies weakly detect IFITM2 in addition to IFITM3. The numbers shown above the graph represent exact p values. Source data are provided as a Source Data file. a Created in BioRender. Denz, P. (2024) BioRender.com/z17v580. Gating strategies for flow cytometry are depicted in Supplementary Fig. a. A549 cells and Supplementary Fig. b. THP-1 cells.

    Article Snippet: HeLa IFITM1/2/3 knockout and HAP1 IFITM3 knockout were purchased from ATCC (CRL-3452) and Horizon Discovery Biosciences (HZGHC004186c010), respectively.

    Techniques: In Vitro, Infection, Flow Cytometry, Control, Western Blot

    a Schematic of mouse passaging experiments. Initial intranasal infections were performed with 1000 TCID50 of parental viruses. b Schematic of WT mouse challenge with parental A/Victoria/361/2011 (H3N2) or passaged viruses. c – h Groups of WT mice ( n = 5 per group) were challenged with 1000 TCID 50 of A/Victoria/361/2011 (H3N2) virus passaged 1, 5, or 10 times through WT or Ifitm3 −/− mice and compared to the parent virus (passage 0). c , f Viral titers from lung homogenates collected at day 7 ( c represents 2 independent infections). d , g ELISA quantification of IL-6. e , h ELISA quantification of IFNβ. c – h Error bars represent SEM and comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Each dot represents an individual mouse. The numbers above the graph represent exact p -values. Source data are provided as a Source Data file. a , b Created in BioRender. Denz, P. (2024) BioRender.com/h97v852.

    Journal: Nature Communications

    Article Title: Innate immune control of influenza virus interspecies adaptation via IFITM3

    doi: 10.1038/s41467-024-53792-3

    Figure Lengend Snippet: a Schematic of mouse passaging experiments. Initial intranasal infections were performed with 1000 TCID50 of parental viruses. b Schematic of WT mouse challenge with parental A/Victoria/361/2011 (H3N2) or passaged viruses. c – h Groups of WT mice ( n = 5 per group) were challenged with 1000 TCID 50 of A/Victoria/361/2011 (H3N2) virus passaged 1, 5, or 10 times through WT or Ifitm3 −/− mice and compared to the parent virus (passage 0). c , f Viral titers from lung homogenates collected at day 7 ( c represents 2 independent infections). d , g ELISA quantification of IL-6. e , h ELISA quantification of IFNβ. c – h Error bars represent SEM and comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. Each dot represents an individual mouse. The numbers above the graph represent exact p -values. Source data are provided as a Source Data file. a , b Created in BioRender. Denz, P. (2024) BioRender.com/h97v852.

    Article Snippet: HeLa IFITM1/2/3 knockout and HAP1 IFITM3 knockout were purchased from ATCC (CRL-3452) and Horizon Discovery Biosciences (HZGHC004186c010), respectively.

    Techniques: Passaging, Virus, Enzyme-linked Immunosorbent Assay

    Influenza virus A/California/04/2009 (H1N1) was passaged through mice as described in Fig. . Groups of WT mice ( n = 5 per group) were challenged with 1000 TCID 50 of A/California/04/2009 (H1N1) virus passaged 1, 5, or 10 times through WT or Ifitm3 −/− mice and compared to the parent virus (passage 0). a , e Viral titers from lung homogenates collected at day 7 ( a ) or day 6 ( e ) post infection. Error bars represent SEM, comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. b Weight loss for the H1N1 series 1 challenge. Error bars represent SEM, comparisons were made using the Mann-Whitney test. ELISA quantification of IL-6 ( c , g ) and IFNβ ( d , h ) levels in lung homogenates of WT and IFITM3 KO mice at day 7 ( c , d ) or day 6 ( g , h ) post infection. Error bars represent SEM and comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. f Weight loss for the H1N1 series 2 challenge. Skull and crossbones indicate humane euthanasia of all animals infected with KO passage 10. Error bars represent SEM, comparisons were made using the Mann-Whitney test. ( a , c , d , e , g , h ) Each dot represents an individual mouse. b , f dots represent averages of individual mice ( n = 5 per group). All numbers above the graphs represent exact p -values. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Innate immune control of influenza virus interspecies adaptation via IFITM3

    doi: 10.1038/s41467-024-53792-3

    Figure Lengend Snippet: Influenza virus A/California/04/2009 (H1N1) was passaged through mice as described in Fig. . Groups of WT mice ( n = 5 per group) were challenged with 1000 TCID 50 of A/California/04/2009 (H1N1) virus passaged 1, 5, or 10 times through WT or Ifitm3 −/− mice and compared to the parent virus (passage 0). a , e Viral titers from lung homogenates collected at day 7 ( a ) or day 6 ( e ) post infection. Error bars represent SEM, comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. b Weight loss for the H1N1 series 1 challenge. Error bars represent SEM, comparisons were made using the Mann-Whitney test. ELISA quantification of IL-6 ( c , g ) and IFNβ ( d , h ) levels in lung homogenates of WT and IFITM3 KO mice at day 7 ( c , d ) or day 6 ( g , h ) post infection. Error bars represent SEM and comparisons were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test. f Weight loss for the H1N1 series 2 challenge. Skull and crossbones indicate humane euthanasia of all animals infected with KO passage 10. Error bars represent SEM, comparisons were made using the Mann-Whitney test. ( a , c , d , e , g , h ) Each dot represents an individual mouse. b , f dots represent averages of individual mice ( n = 5 per group). All numbers above the graphs represent exact p -values. Source data are provided as a Source Data file.

    Article Snippet: HeLa IFITM1/2/3 knockout and HAP1 IFITM3 knockout were purchased from ATCC (CRL-3452) and Horizon Discovery Biosciences (HZGHC004186c010), respectively.

    Techniques: Virus, Infection, MANN-WHITNEY, Enzyme-linked Immunosorbent Assay